Anticorps Monoclonal anti-TRPV1

TRPV1 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

22

CloneNo.

1A3C9

Avis (1)

N° de cat : 66983-1-Ig

Synonymes

Capsaicin receptor, DKFZp434K0220, Osm 9 like TRP channel 1, OTRPC1, TRPV1, Vanilloid receptor 1, VR1

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Galerie de données de validation

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  WB analysis of HepG2 using 66983-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-468 using 66983-1-Ig

  MDA-MB-468 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 66983-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66983-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 66983-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 66983-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 66983-1-Ig (TRPV1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HEK-293, cellules HUVEC, cellules MDA-MB-468, cellules T-47D, cellules Y79

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below

Informations sur le produit

66983-1-Ig cible TRPV1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TRPV1 Protéine recombinante Ag18683
• Nom complet: transient receptor potential cation channel, subfamily V, member 1
• Masse moléculaire calculée: 839 aa, 95 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC132820
• Symbole du gène: TRPV1
• Identification du gène (NCBI): 7442
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPV1, also named as VR1 or OTRPC1, belongs to the transient receptor potential (TRP) family and TRPV subfamily (PMID: 17579562). TRPV1 is a ligand-activated non-selective calcium permeant cation channel expressed predominantly by sensory neurons (PMID: 10821274). It is involved in detection of noxious chemical and thermal stimuli. TRPV1 plays an important role in inflammatory thermal hyperalgesia (PMID: 10764638; 10821274).

Publications

Species	Application	Title
		Int J Mol Sci; The Contribution of TSLP Activation to Hyperalgesia in Dorsal Root Ganglia Neurons of a Rat.; Authors - Chun-Ching Lu
	WB	Biochim Biophys Acta Mol Basis Dis; Palmitic acid and trans-4-hydroxy-3-methoxycinnamate, the active ingredients of Yaobishu formula, reduce inflammation and pain by regulating gut microbiota and metabolic changes after lumbar disc herniation to activate autophagy and the Wnt/β-catenin pathway; Authors - ShuoQi Li
human	WB	EBioMedicine; Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis; Authors - Zhongyang Lv
human	WB	Int J Mol Sci; Luteolin Alleviates Oxidative Stress in Chronic Obstructive Pulmonary Disease Induced by Cigarette Smoke via Modulation of the TRPV1 and CYP2A13/NRF2 Signaling Pathways; Authors - Lina Zhou
human	WB	J Inflamm Res; Cannabidiol Inhibits Inflammation Induced by Cutibacterium acnes-Derived Extracellular Vesicles via Activation of CB2 Receptor in Keratinocytes; Authors - Ziqi Jiang
human	WB	Cell Calcium; Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury.; Authors - Zhenhong Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Shihab (Verified Customer) (09-17-2021)

This antibody was tried in a cell type (cannot disclose) which has previously been shown to express plenty of TRPV1 signal. However, this antibody was unable to give any signal in various attempts at staining, even at 1:100 dilution. This is not to say that this anybody is not good because I have had trouble with various TRPV1 antibodies in this tissue type but it will be used in other cell/tissue type to see its staining capabilities

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100