Anticorps Polyclonal de lapin anti-TRPV4

TRPV4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 27892-1-AP

Synonymes

Osm 9 like TRP channel 4, OTRPC4, TRP12, TRPV4, VR OAC, VRL 2, VRL2, VROAC

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Galerie de données de validation

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  WB analysis using 27892-1-AP

  SH-SY5Y cell lysates (heated at 100℃, heated at 37℃) were subjected to SDS PAGE followed by western blot with 27892-1-AP (TRPV4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: 37°C incubated SH-SY5Y cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

27892-1-AP cible TRPV4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPV4 Protéine recombinante Ag27253
• Nom complet: transient receptor potential cation channel, subfamily V, member 4
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 85~98 kDa
• Numéro d’acquisition GenBank: NM_001177428.1
• Symbole du gène: TRPV4
• Identification du gène (NCBI): 59341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPV4, also named as VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.

Publications

Species	Application	Title
rat	WB	Front Med (Lausanne); Low hydrostatic pressure promotes functional homeostasis of nucleus pulposus cells through the TRPV4/CRT/FA complex axis; Authors - Junxian Hu

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