Anticorps Recombinant de lapin anti-TRPV4

TRPV4 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241143H1

N° de cat : 84091-4-PBS

Synonymes

Vanilloid receptor-like channel 2, TRP12, OTRPC4, Osm-9-like TRP channel 4, Osm 9 like TRP channel 4

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Galerie de données de validation

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  WB analysis using 84091-4-RR (same clone as 84091-4-PBS)

  Rat brain tissue(heated at 100 ° C and unboilded)lysates were subjected to SDS PAGE followed by western blot with 84091-4-RR (TRPV4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84091-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84091-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84091-4-RR against Human TRPV4 were performed. The affinity constant is 2.57 nM.

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Informations sur le produit

84091-4-PBS cible TRPV4 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRPV4 Protéine recombinante Ag27253
• Nom complet: transient receptor potential cation channel, subfamily V, member 4
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 85~98 kDa
• Numéro d’acquisition GenBank: NM_001177428.1
• Symbole du gène: TRPV4
• Identification du gène (NCBI): 59341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TRPV4, also called VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.

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