Anticorps Recombinant de lapin anti-TRPV4

TRPV4 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241143H1

N° de cat : 84091-4-RR

Synonymes

Vanilloid receptor-like channel 2, TRP12, OTRPC4, Osm-9-like TRP channel 4, Osm 9 like TRP channel 4

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Galerie de données de validation

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  WB analysis using 84091-4-RR

  Rat brain tissue(heated at 100 ° C and unboilded)lysates were subjected to SDS PAGE followed by western blot with 84091-4-RR (TRPV4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84091-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84091-4-RR against Human TRPV4 were performed. The affinity constant is 2.57 nM.

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Applications testées

• Résultats positifs en WB: unboiled rat brain tissue,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84091-4-RR cible TRPV4 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TRPV4 Protéine recombinante Ag27253
• Nom complet: transient receptor potential cation channel, subfamily V, member 4
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 85~98 kDa
• Numéro d’acquisition GenBank: NM_001177428.1
• Symbole du gène: TRPV4
• Identification du gène (NCBI): 59341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TRPV4, also called VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.

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