Anticorps Monoclonal anti-Tuberin/TSC2

• Phare
• Validé par KD/KO

Tuberin/TSC2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A10B2

N° de cat : 68380-1-PBS

Synonymes

TSC2, Tuberin, 1A10B2, TSC4, tuberous sclerosis 2

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Galerie de données de validation

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  WB analysis using 68380-1-Ig (same clone as 68380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68380-1-Ig (same clone as 68380-1-PBS)

  WB result of Tuberin/TSC2 antibody (68380-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 68380-1-Ig (same clone as 68380-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 68380-1-Ig (same clone as 68380-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 68380-1-Ig (same clone as 68380-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68380-1-Ig (same clone as 68380-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68380-1-Ig (TSC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68380-1-Ig (Tuberin/TSC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 68380-1-Ig (same clone as 68380-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TSC2 antibody (68380-1-Ig, Clone: 1A10B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68380-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68380-1-PBS cible Tuberin/TSC2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tuberous sclerosis 2
• Masse moléculaire calculée: 1807 aa, 201 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC150300
• Symbole du gène: TSC2
• Identification du gène (NCBI): 7249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.

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