Anticorps Polyclonal de lapin anti-TSC22D1

• Phare
• Validé par KD/KO

TSC22D1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 10214-1-Ig

Synonymes

Cerebral protein 2, KIAA1994, Regulatory protein TSC 22, RP11 269C23.2, TGFB1I4, TSC22, TSC22 domain family protein 1, TSC22 domain family, member 1, TSC22D1

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Galerie de données de validation

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  WB analysis of human brain using 10214-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10214-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10214-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 10214-1-Ig

  IP Result of anti-TSC22D1 (IP:10214-1-Ig, 4ug; Detection:10214-1-Ig 1:500) with rat brain tissue lysate 4000ug.

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  IHC staining of human testis using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 10214-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10214-1-Ig (TSC22D1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A549 using 10214-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed A549 cells using 10214-1-Ig(TSC22D1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules A549, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

10214-1-Ig cible TSC22D1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSC22D1 Protéine recombinante Ag0287
• Nom complet: TSC22 domain family, member 1
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC000456
• Symbole du gène: TSC22D1
• Identification du gène (NCBI): 8848
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSC22 domain family 1, transforming growth factor beta 1 induced transcript 4, (TSC22D1, synonyms: TSC22, TGFB1I4, MGC17597) is a member of a family of leucine zipper containing transcription factors with repressor activity and belongs to the large family of early response genes. TSC-22 regulates cell growth and differentiation, and cell death. Leucine zipper structure of TSC22 inhibits the anchorage independent growth of cancer cells. TSC-22 is also an important downstream component of peroxisome proliferator-activated receptor (PPAR) and TGF-beta signaling during intestinal epithelial cell differentiation. This antibody is a rabbit polyclonal antibody raised against an internal region of human TSC22D1.

Publications

Species	Application	Title
mouse	WB	Cell Death Differ; TSC-22D1 isoforms have opposing roles in mammary epithelial cell survival.; Authors - Huser C A CA
human,mouse	WB,IHC	Clin Cancer Res; Transforming Growth Factor-{beta}-Stimulated Clone-22 Is an Androgen-Regulated Gene That Enhances Apoptosis in Prostate Cancer following Insulin-Like Growth Factor-I Receptor Inhibition.; Authors - Sprenger Cynthia C T CC ; KD Validated
human	WB,IF	Int J Mol Sci; Identification of Binding Proteins for TSC22D1 Family Proteins Using Mass Spectrometry.; Authors - Ryouta Kamimura
mouse	IP,WB	FEBS J; Multiple TSC22D4 iso-/phospho-glycoforms display idiosyncratic subcellular localizations and interacting protein partners.; Authors - Canterini Sonia S
human	WB,IF	Am J Physiol Heart Circ Physiol; Transforming growth factor-beta1 regulation of C-type natriuretic peptide expression in human vascular smooth muscle cells: dependence on TSC22D1.; Authors - Mendon?a Maria C MC
human	WB,IHC	Biochem Biophys Res Commun; Low levels of TSC22 enhance tumorigenesis by inducing cell proliferation in colorectal cancer.; Authors - Yong Zhou