Anticorps Polyclonal de lapin anti-TSEN34

• Phare
• Validé par KD/KO

TSEN34 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31212-1-PBS

Synonymes

EC:4.6.1.16, HsSen34, LENG5, PCH2C, SEN34

---

Galerie de données de validation

• 

  WB analysis using 31212-1-AP (same clone as 31212-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31212-1-AP (TSEN34 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31212-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 31212-1-AP (same clone as 31212-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31212-1-AP (TSEN34 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31212-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 31212-1-AP (same clone as 31212-1-PBS)

  WB result of TSEN34 antibody (31212-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSEN34 transfected MCF-7 cells. This data was developed using the same antibody clone with 31212-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 31212-1-AP (same clone as 31212-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TSEN34 antibody (31212-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31212-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

31212-1-PBS cible TSEN34 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSEN34 Protéine recombinante Ag35120
• Nom complet: tRNA splicing endonuclease 34 homolog (S. cerevisiae)
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34-36 kDa
• Numéro d’acquisition GenBank: BC020805
• Symbole du gène: TSEN34
• Identification du gène (NCBI): 79042
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The human tRNA splicing endonuclease consists of a heterotetramer complex formed by the four TSEN proteins, which were identified by homology with their yeast counterparts. TSEN2 and TSEN34 are the catalytic subunits, which form a compound active site, whereas TSEN54 and TSEN15 are structural proteins. (PMID: 27392077)

{{ptg:RelatedPrimaryAntibodies}}