Anticorps Monoclonal anti-TSG101

TSG101 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

2B7G8

N° de cat : 67381-1-PBS

Synonymes

2B7G8, ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10

---

Galerie de données de validation

• 

  WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67381-1-Ig (same clone as 67381-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human urine exosomes using 67381-1-Ig (same clone as 67381-1-PBS)

  human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67381-1-Ig (TSG101 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using TSG101 antibody (67381-1-Ig, Clone: 2B7G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67381-1-Ig (TSG101 antibody), at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67381-1-Ig (same clone as 67381-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug TSG101 Monoclonal antibody (67381-1-Ig, Clone:2B7G8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67381-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67381-1-PBS cible TSG101 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TSG101 Protéine recombinante Ag28569
• Nom complet: tumor susceptibility gene 101
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 43-46 kDa
• Numéro d’acquisition GenBank: BC002487
• Symbole du gène: TSG101
• Identification du gène (NCBI): 7251
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

{{ptg:RelatedPrimaryAntibodies}}