Anticorps Recombinant de lapin anti-TSG101

TSG101 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241657A1

N° de cat : 84334-4-PBS

Synonymes

241657A1, ESCRT-I complex subunit TSG101, TSG 10, TSG 101, TSG10

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Galerie de données de validation

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  WB analysis using 84334-4-RR (same clone as 84334-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84334-4-RR (TSG101 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84334-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84334-4-RR (same clone as 84334-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84334-4-RR (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84334-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84334-4-RR (same clone as 84334-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSG101 antibody (84334-4-RR, Clone: 241657A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84334-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84334-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84334-4-RR against Human TSG101 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84334-4-PBS cible TSG101 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TSG101 Protéine recombinante Ag28569
• Nom complet: tumor susceptibility gene 101
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC002487
• Symbole du gène: TSG101
• Identification du gène (NCBI): 7251
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

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