Anticorps Monoclonal anti-TSG101

TSG101 Monoclonal Antibody for IF-P, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

IF-P, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(1)

CloneNo.

2B7G8

N° de cat : CL488-67381

Synonymes

VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101

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Galerie de données de validation

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  IF Staining of human colon cancer using CL488-67381

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL488-67381

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite® Plus 488-conjugated TSG101 antibody (CL488-67381, Clone: 2B7G8 ) at dilution of 1:200.

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  FC experiment of THP-1 using CL488-67381

  1X10^6 THP-1 cells were stained with 0.2 ug CoraLite® Plus 488 Anti-Human TSG101 (CL488-67381, Clone: 2B7G8) (red), or 0.2 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du côlon humain,
• Résultats positifs en FC (Intra): cellules THP-1

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 1 publications below

Informations sur le produit

CL488-67381 cible TSG101 dans les applications de IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TSG101 Protéine recombinante Ag28569
• Nom complet: tumor susceptibility gene 101
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 43-46 kDa
• Numéro d’acquisition GenBank: BC002487
• Symbole du gène: TSG101
• Identification du gène (NCBI): 7251
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Publications

Species	Application	Title
goat	FC	Comp Biochem Physiol Part D Genomics Proteomics; Exosomes serve as a crucial mediator of epithelial-fibroblast communication during hair follicle morphogenesis in cashmere goats; Authors - Erhan Hai

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