Anticorps Polyclonal de lapin anti-TSPAN2

TSPAN2 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20463-1-AP

Synonymes

6330415F13Rik, NET3, Tetraspan NET 3, tetraspanin 2, TSN2, TSPAN 2, TSPAN2

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Galerie de données de validation

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  WB analysis using 20463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20463-1-AP (TSPAN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 20463-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20463-1-AP (TSPAN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HL-60 using 20463-1-AP

  IP Result of anti-TSPAN2 (IP:20463-1-AP, 4ug; Detection:20463-1-AP 1:500) with HL-60 cells lysate 960ug.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules K-562
• Résultats positifs en IP: cellules HL-60

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20463-1-AP cible TSPAN2 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSPAN2 Protéine recombinante Ag14299
• Nom complet: tetraspanin 2
• Masse moléculaire calculée: 221 aa, 24 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC021675
• Symbole du gène: TSPAN2
• Identification du gène (NCBI): 10100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSPAN2 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. It has been reported that TSPAN2 is involved in cell invasion and motility during lung cancer progression (PMID: 24726368). TSPAN2 enhances cell motility and invasiveness by assisting CD44 in scavenging intracellular reactive oxygen species.

Publications

Species	Application	Title
human,mouse	WB	Elife; Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice.; Authors - Vasiliki-Ilya Gargareta
human	IF	FASEB J; Selective expression of TSPAN2 in vascular smooth muscle is independently regulated by TGF-β1/SMAD and myocardin/serum response factor.; Authors - Jinjing Zhao