Anticorps Polyclonal de lapin anti-TSPAN9

TSPAN9 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 21983-1-AP

Synonymes

NET 5, NET5, PP1057, Tetraspan NET 5, tetraspanin 9, Tspan 9, TSPAN9

---

Galerie de données de validation

• 

  WB analysis of mouse peripheral blood leukocyte using 21983-1-AP

  mouse peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 21983-1-AP (TSPAN9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21983-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21983-1-AP (TSPAN9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 21983-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 21983-1-AP (TSPAN9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 21983-1-AP

  IP Result of anti-TSPAN9 (IP:21983-1-AP, 4ug; Detection:21983-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

---

Applications testées

• Résultats positifs en WB: leucocyte de sang périphérique de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• FC: See 1 publications below

Informations sur le produit

21983-1-AP cible TSPAN9 dans les applications de WB, IP, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSPAN9 Protéine recombinante Ag17048
• Nom complet: tetraspanin 9
• Masse moléculaire calculée: 239 aa, 27 kDa
• Poids moléculaire observé: 29-33 kDa
• Numéro d’acquisition GenBank: BC071881
• Symbole du gène: TSPAN9
• Identification du gène (NCBI): 10867
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSPAN9 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN9 is relatively highly expressed in the megakaryocyte/platelet lineage and has a role in regulating platelet function in concert with other platelet tetraspanins and their associated proteins. TSPAN9 has been identified as a glycoprotein (PMID: 18795891). This polyclonal antibody detects endogenous TSPAN9 at a band of 29-33 kDa, which is larger than the calculated molecular weight of TSPAN9 (27 kDa), probably due to glycosylation.

Publications

Species	Application	Title
human,mouse,rat	WB,FC	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li