Anticorps Polyclonal de lapin anti-CDA1

• Phare
• Validé par KD/KO

CDA1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 12087-2-AP

Synonymes

CDA1, Cell division autoantigen 1, CINAP, CTCL, DENTT, NP79, Nuclear protein of 79 kDa, TSPX, TSPYL2

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Galerie de données de validation

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  WB analysis of HeLa using 12087-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12087-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12087-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 12087-2-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12087-2-AP

  IP Result of anti-CDA1 (IP:12087-2-AP, 4ug; Detection:12087-2-AP 1:1000) with HEK-293 cells lysate 1000ug.

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  IHC staining of human lung cancer using 12087-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12087-2-AP (CDA1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12087-2-AP

  Immunofluorescent analysis of Hela cells, using TSPYL2 antibody 12087-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules DU 145, cellules HEK-293, cellules MCF-7
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12087-2-AP cible CDA1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CDA1 Protéine recombinante Ag2722
• Nom complet: TSPY-like 2
• Masse moléculaire calculée: 693 aa, 79 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC024270
• Symbole du gène: TSPYL2
• Identification du gène (NCBI): 64061
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSPYL2 (also known as CINAP, CDA1, TSPX or DENTT) is a new member of the nucleosome assembly protein superfamily. TSPYL2 binds histones and facilitates nucleosome assembly. TSPYL2 is expressed in various tissues, highly in the pituitary gland and moderately in the adrenals, brain, testis, and ovary. Immunohistochemical staining analysis for TSPYL2 showed differential cytoplasmic and nuclear staining patterns in several cell types. Downregulated expression of TSPYL2 has been observed in several tumors, which suggests its role as a tumor suppressor. Although it is predicted that TSPYL2 has a molecular mass of 79.43 kDa, it is found that mammalian TSPYL2 appears at a size of 120 kDa by western blot analysis. The abundant acidic amino acid regions in TSPYL2 may cause its aberrant migration. In addition, the TSPYL2 protein is unstable and sensitive to proteasomal degradation.

Publications

Species	Application	Title
human	WB	Cell; Glioma stem cell proliferation and tumor growth are promoted by nitric oxide synthase-2.; Authors - Eyler Christine E CE
human	WB	Cell Death Differ; TSPYL2 is an essential component of the REST/NRSF transcriptional complex for TGFβ signaling activation.; Authors - M T Epping ; KD Validated
human	WB	PLoS One; The X-linked tumor suppressor TSPX interacts and promotes degradation of the hepatitis B viral protein HBx via the proteasome pathway.; Authors - Kido Tatsuo T
human	IF	Stem Cells Int; Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells.; Authors - Sabine Conrad
human	WB	Oncotarget; The X-linked tumor suppressor TSPX downregulates cancer-drivers/oncogenes in prostate cancer in a C-terminal acidic domain dependent manner.; Authors - Tatsuo Kido
human	WB,IF	J Ginseng Res; Ginsenoside Rg3 increases gemcitabine sensitivity of pancreatic adenocarcinoma via reducing ZFP91 mediated TSPYL2 destabilization; Authors - Haixia Pan