Anticorps Polyclonal de lapin anti-TST

• Phare
• Validé par KD/KO

TST Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 16311-1-AP

Synonymes

Rhodanese, RDS, EC:2.8.1.1

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Galerie de données de validation

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  WB analysis of liver tissue using 16311-1-AP

  WB result of anti-TST (16311-1-AP) in Liver by Dr. Seow Chong Lee.

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  WB analysis using 16311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16311-1-AP (TST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16311-1-AP

  WB result of TST antibody (16311-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TST transfected HEK-293 cells.

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  WB analysis of human kidney using 16311-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16311-1-AP (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16311-1-AP

  IP result of anti-TST (IP:16311-1-AP, 4ug; Detection:16311-1-AP 1:300) with HEK-293 cells lysate 2800ug.

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  IHC staining of human colon cancer using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16311-1-AP (TST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16311-1-AP (TST antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16311-1-AP (TST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 16311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16311-1-AP (TST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, rein de rat, rein de souris, tissu rénal humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below

Informations sur le produit

16311-1-AP cible TST dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TST Protéine recombinante Ag9346
• Nom complet: thiosulfate sulfurtransferase (rhodanese)
• Masse moléculaire calculée: 297 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC010148
• Symbole du gène: TST
• Identification du gène (NCBI): 7263
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.

Publications

Species	Application	Title
human	WB	EMBO Mol Med; Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway.; Authors - Marcello Ziosi
mouse	WB	EMBO Mol Med; CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.; Authors - Marta Luna-Sánchez
mouse	WB	Antioxid Redox Signal; The Cysteine Dioxgenase Knockout Mouse: Altered Cysteine Metabolism in Nonhepatic Tissues Leads to Excess H2S/HS(-) Production and Evidence of Pancreatic and Lung Toxicity.; Authors - Roman Heather B HB
mouse,human	WB	Hum Mol Genet; Coenzyme Q10 modulates sulfide metabolism and links the mitochondrial respiratory chain to pathways associated to one carbon metabolism.; Authors - Pilar González-García
mouse	WB	Biochem Pharmacol; Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis.; Authors - Katalin Módis
mouse	WB	Amino Acids; Primary hepatocytes from mice lacking cysteine dioxygenase show increased cysteine concentrations and higher rates of metabolism of cysteine to hydrogen sulfide and thiosulfate.; Authors - Halina Jurkowska

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