Anticorps Polyclonal de lapin anti-TTBK1

TTBK1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 32938-1-AP

Synonymes

BDTK, Brain-derived tau kinase, EC:2.7.11.1, KIAA1855, Tau-tubulin kinase 1

---

Galerie de données de validation

• 

  WB analysis using 32938-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32938-1-AP (TTBK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal

Dilution recommandée

• Western Blot (WB): WB : 1:6000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32938-1-AP cible TTBK1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TTBK1 Protéine recombinante Ag36655
• Nom complet: tau tubulin kinase 1
• Masse moléculaire calculée: 142/70 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC036764
• Symbole du gène: TTBK1
• Identification du gène (NCBI): 84630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TTBK1 (Tau Tubulin kinase 1), also known as BDTK (brain-derived Tau kinase), is a 1,321 amino acid protein that contains one protein kinase domain and belongs to the serine/threonine protein kinase family. Localized to the cytoplasm and expressed at high levels in brain and at lower levels in testis and spinal cord, TTBK1 functions as a serine/threonine kinase that can phosphorylate Tau (a protein involved in Tubulin polymerization) on threonine, tyrosine and serine residues. Specifically, TTBK1 uses divalent cations, such as magnesium and manganese, to catalyze the ATP-dependent transfer of a phosphate group onto Tau, creating a phosphoprotein and ADP. Phosphorylation of Tau causes its aggregation and subsequent loss of function, suggesting an important role for TTBK1 in the control of Tubulin dynamics. Two isoforms of TTBK1 are expressed due to alternative splicing events. The molecular weight observed is consistent with what has been described in PMID:16923168.

{{ptg:RelatedPrimaryAntibodies}}