Anticorps Polyclonal de lapin anti-TTBK2

• Phare
• Validé par KD/KO

TTBK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (1)

N° de cat : 15072-1-AP

Synonymes

KIAA0847, SCA11, tau tubulin kinase 2, TTBK, TTBK2

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Galerie de données de validation

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  WB analysis of PC-3 using 15072-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15072-1-AP (TTBK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15072-1-AP

  WB result of TTBK2 antibody (15072-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTBK2 transfected HeLa cells.

• 

  WB analysis of SH-SY5Y using 15072-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15072-1-AP (TTBK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15072-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 15072-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 15072-1-AP (TTBK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 6 publications below

Informations sur le produit

15072-1-AP cible TTBK2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TTBK2 Protéine recombinante Ag7108
• Nom complet: tau tubulin kinase 2
• Masse moléculaire calculée: 137 kDa
• Poids moléculaire observé: 137-150 kDa
• Numéro d’acquisition GenBank: BC071556
• Symbole du gène: TTBK2
• Identification du gène (NCBI): 146057
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TTBK2(tau tubulin kinase 2) plays an important role in the tau cascade and in spinocerebellar degeneration. The N-terminus of TTBK2 has a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK1 and TTBK2 function as regulators of TDP-43 phosphorylation. TTBK1/2 may be attractive drug targets for therapeutic interventions in TDP-43 proteinopathies such as FTLD-TDP and ALS.(PMID:25473830). 15072-1-AP has a cross reaction with TTBK1.

Publications

Species	Application	Title
mouse	WB,IF	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human	WB	PLoS Genet; The Tau Tubulin Kinases TTBK1/2 Promote Accumulation of Pathological TDP-43.; Authors - Nicole F Liachko
human	IF	Sci Rep; MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment.; Authors - Si-Hyung Lee
human	IF	Hum Mol Genet; Altered gene regulation as a candidate mechanism by which ciliopathy gene SDCCAG8 contributes to schizophrenia and cognitive function.; Authors - Mairéad Flynn
human	IF	J Cell Sci; Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair.; Authors - Ebtissal M Khouj
mouse	IF	PLoS Genet; Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.; Authors - Emily Bowie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (01-27-2023)

Used on lysates from HEK293T cells. Incubation for a few hours at room temperature. Detection of 2 main bands, 1 above 118 kDa marker (expected size of TTBK2 is 137-150 kDa) and 1 between 47 and 85 kDa markers.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T