Anticorps Polyclonal de lapin anti-TTC26

TTC26 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 25083-1-AP

Synonymes

IFT56, dyf 13

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Galerie de données de validation

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  WB analysis of mouse testis using 25083-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25083-1-AP (TTC26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25083-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25083-1-AP (TTC26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 25083-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25083-1-AP (TTC26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 25083-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using TTC26 antibody (25083-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HEK-293, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules hTERT-RPE1,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

25083-1-AP cible TTC26 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TTC26 Protéine recombinante Ag18983
• Nom complet: tetratricopeptide repeat domain 26
• Masse moléculaire calculée: 554 aa, 64 kDa
• Poids moléculaire observé: 60-64 kDa
• Numéro d’acquisition GenBank: BC126331
• Symbole du gène: TTC26
• Identification du gène (NCBI): 79989
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TTC26 (Tetratricopeptide repeat protein 26), also known as IFT56 (Intraflagellar transport protein 56), is an IFT complex B protein, which has a role in transport of ciliary cargo, important for ciliary motility and construction of full length cilia (PMID: 38135897). Severe biliary ciliopathy, caused by bi-allelic mutations in TTC26, has been recently described in the context of a syndrome of polydactyly and severe neonatal cholestasis, with brain, kidney and heart involvement (PMID: 32617964).

Publications

Species	Application	Title
human	WB	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie

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