Anticorps Polyclonal de lapin anti-TTDN1

TTDN1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25410-1-PBS

Synonymes

C7orf11, ABHS, TTD non-photosensitive 1 protein, ORF20, MPLKIP

---

Galerie de données de validation

• 

  WB analysis of NIH/3T3 using 25410-1-AP (same clone as 25410-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25410-1-AP (TTDN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 25410-1-AP (same clone as 25410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25410-1-AP (TTDN1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25410-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

25410-1-PBS cible TTDN1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TTDN1 Protéine recombinante Ag22094
• Nom complet: chromosome 7 open reading frame 11
• Masse moléculaire calculée: 179 aa, 19 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC026265
• Symbole du gène: TTDN1
• Identification du gène (NCBI): 136647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TTDN1 colocalizes with Plk1 at the centrosome in mitosis and the midbody during cytokinesis. It is phosphorylated by Cdk1 in mitosis, and this is required for its interaction with Plk1. Overexpression of TTDN1 or its knockdown by siRNA causes multi-polar spindles and multiple nuclei, suggesting that TTDN1 plays a role in regulating mitosis and cytokinesis. Mutations in the TTDN1 gene are associated with a distinct trichothiodystrophy phenotype. (PMID:17310276, 25290684)

{{ptg:RelatedPrimaryAntibodies}}