Anticorps Monoclonal anti-TTF1/NKX2-1

TTF1/NKX2-1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain, porc

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2C8F3

N° de cat : 66034-1-PBS

Synonymes

TTF1, TTF1; NKX2-1, 2C8F3, BHC, Homeobox protein Nkx 2.1

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Galerie de données de validation

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  WB analysis of HepG2 using 66034-1-Ig (same clone as 66034-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66034-1-Ig (TTF1/NKX2-1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1/NKX2-1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66034-1-Ig (same clone as 66034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1/NKX2-1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1/NKX2-1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1/NKX2-1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1/NKX2-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 66034-1-Ig (same clone as 66034-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1/NKX2-1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 66034-1-Ig (same clone as 66034-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using TTF1 antibody (66034-1-Ig, Clone: 2C8F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66034-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66034-1-PBS cible TTF1/NKX2-1 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: NK2 homeobox 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: NM_003317
• Symbole du gène: TTF1
• Identification du gène (NCBI): 7080
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Homeobox protein Nkx-2.1 (NKX2-1), also named Thyroid nuclear factor 1,Thyroid transcription factor 1(TTF1). It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD).

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