Anticorps Polyclonal de lapin anti-TTL

• Phare
• Validé par KD/KO

TTL Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(34)

Avis (1)

N° de cat : 13618-1-AP

Synonymes

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25

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Galerie de données de validation

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  WB analysis of HeLa using 13618-1-AP

  WB result of TTL antibody (13618-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTL transfected HeLa cells.

• 

  WB analysis using 13618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13618-1-AP (TTL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13618-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13618-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13618-1-AP (TTL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 13618-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13618-1-AP (TTL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 13618-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 13618-1-AP (TTL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cerveau de rat, cerveau de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 29 publications below
• IF: See 6 publications below

Informations sur le produit

13618-1-AP cible TTL dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TTL Protéine recombinante Ag4526
• Nom complet: tubulin tyrosine ligase
• Masse moléculaire calculée: 377 aa, 43 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC036819
• Symbole du gène: TTL
• Identification du gène (NCBI): 150465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

Publications

Species	Application	Title
	WB	Science; Posttranslational modification of microtubules by the MATCAP detyrosinase.; Authors - Lisa Landskron
mouse	WB	Nature; MARK4 controls ischaemic heart failure through microtubule detyrosination.; Authors - Xian Yu
human	WB	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
human	WB	Science; Vasohibins encode tubulin detyrosinating activity.; Authors - Joppe Nieuwenhuis
human	WB	Science; Mitosis. Microtubule detyrosination guides chromosomes during mitosis.; Authors - Marin Barisic
human	WB	Nat Med; Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure.; Authors - Christina Yingxian Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

Multiple bands corresponding to different isoforms.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart

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