Anticorps Monoclonal anti-TTL

• Phare
• Validé par KD/KO

TTL Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E5F8

N° de cat : 66076-1-PBS

Synonymes

2E5F8, EC:6.3.2.25, Tubulin tyrosine ligase, tubulin tyrosine ligase, Tubulin--tyrosine ligase

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Galerie de données de validation

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  WB analysis using 66076-1-Ig (same clone as 66076-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66076-1-Ig (same clone as 66076-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66076-1-Ig (same clone as 66076-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66076-1-Ig (TTL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66076-1-Ig (same clone as 66076-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66076-1-Ig(TTL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66076-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66076-1-PBS cible TTL dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TTL Protéine recombinante Ag4708
• Nom complet: tubulin tyrosine ligase
• Masse moléculaire calculée: 377 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC036819
• Symbole du gène: TTL
• Identification du gène (NCBI): 150465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

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