Anticorps Polyclonal de lapin anti-TTRAP

• Phare
• Validé par KD/KO

TTRAP Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 12203-1-AP

Synonymes

EAP2, AD-022, AD022, 5'-tyrosyl-DNA phosphodiesterase, 5'-Tyr-DNA phosphodiesterase

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Galerie de données de validation

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  WB analysis of human spleen using 12203-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12203-1-AP (TTRAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12203-1-AP

  WB result of TTRAP antibody (12203-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTRAP transfected HepG2 cells.

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  WB analysis using 12203-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12203-1-AP (TTRAP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12203-1-AP

  IP result of anti-TTRAP (IP:12203-1-AP, 4ug; Detection:12203-1-AP 1:5000) with HepG2 cells lysate 1600 ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, tissu splénique humain
• Résultats positifs en IP: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

12203-1-AP cible TTRAP dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TTRAP Protéine recombinante Ag2846
• Nom complet: TRAF and TNF receptor associated protein
• Masse moléculaire calculée: 362 aa, 41 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC017553
• Symbole du gène: TTRAP
• Identification du gène (NCBI): 51567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TTRAP is a 5-prime tyrosyl-DNA phosphodiesterase that is required for efficient repair of topoisomerase II-induced DNA double-strand breaks. Thus it can remove lots of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, generating a free 5' phosphate to DNA. It also has 3'-tyrosyl DNA phophodiesterase activity, but not so efficient as TDP1. TTRAP functions as a negative regulator of ETS1 and inhibit nuclear factor-kappa-B activation.

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res; Reversal of DNA damage induced Topoisomerase 2 DNA-protein crosslinks by Tdp2.; Authors - Matthew J Schellenberg
mouse	WB	Cell Death Differ; NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.; Authors - Bin Zhang ; KO Validated
human	WB	Anal Biochem; Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.; Authors - Graeme Thomson
human	WB	Int J Mol Sci; Combined PARP and Dual Topoisomerase Inhibition Potentiates Genome Instability and Cell Death in Ovarian Cancer; Authors - Inken Flörkemeier
mouse	WB	bioRxiv; DNA polymerase theta-mediated DNA repair is a functional dependency and therapeutic vulnerability in DNMT3A deficient leukemia cells; Authors - Bac Viet Le

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