Anticorps Recombinant de lapin anti-TUBB3-specific/TUJ1

TUBB3-specific/TUJ1 Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250851G10

N° de cat : 80128-3-PBS

Synonymes

beta tubulin, Tubulin beta 3 chain, Tubulin beta III, TUBB3, TUBB4

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Galerie de données de validation

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  WB analysis using 80128-3-RR (same clone as 80128-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80128-3-RR (TUBB3-specific antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 80128-3-RR (TUBB3-specific/TUJ1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 80128-3-RR (TUBB3-specific/TUJ1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse cerebellum using 80128-3-RR (same clone as 80128-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using TUBB3-specific antibody (80128-3-RR, Clone: 250851G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80128-3-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP00550-2

  The mean TUBB3 concentration was determined to be 6.4 ng/mL in SH-SY5Y cell extract based on a 1.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP00550-2

  Cytometric bead array standard curve of MP00550-2, TUBB3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80128-3-PBS. Detection antibody: 80128-2-PBS. Standard: SY01676. Range: 0.039-2.5 ng/mL.

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Informations sur le produit

80128-3-PBS cible TUBB3-specific/TUJ1 dans les applications de WB, IHC, IF-P, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tubulin, beta 3
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: NM_001197181
• Symbole du gène: TUBB3
• Identification du gène (NCBI): 10381
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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