Anticorps Polyclonal de lapin anti-TUBG2

TUBG2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28009-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 28009-1-AP (same clone as 28009-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.

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  WB analysis using 28009-1-AP (same clone as 28009-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 28009-1-AP (same clone as 28009-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28009-1-AP (TUBG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 28009-1-AP (same clone as 28009-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TUBG2 antibody (28009-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 28009-1-AP (same clone as 28009-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUBG2 (28009-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 28009-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28009-1-PBS cible TUBG2 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TUBG2 Protéine recombinante Ag27728
• Nom complet: tubulin, gamma 2
• Masse moléculaire calculée: 451 aa, 51 kDa
• Poids moléculaire observé: 51 kDa
• Numéro d’acquisition GenBank: BC051890
• Symbole du gène: TUBG2
• Identification du gène (NCBI): 27175
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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