Anticorps Monoclonal anti-TUFM

TUFM Monoclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2A10D2

Avis (1)

N° de cat : 67802-1-Ig

Synonymes

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

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Galerie de données de validation

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  WB analysis using 67802-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67802-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 67802-1-Ig

  IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug.

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  IHC staining of human colon cancer using 67802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67802-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules 4T1, cellules HCT 116, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules K-562, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67802-1-Ig cible TUFM dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TUFM Protéine recombinante Ag24976
• Nom complet: Tu translation elongation factor, mitochondrial
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC010041
• Symbole du gène: TUFM
• Identification du gène (NCBI): 7284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Publications

Species	Application	Title
mouse	WB	Mol Ther Nucleic Acids; Decoding CKD-induced muscle atrophy through the critical role of lncRNA GAS5 and pyroptosis; Authors - Rong Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah-Eve (Verified Customer) (02-19-2023)

Works very Well for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Human pulmonary artery smooth muscle cells

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