Anticorps Monoclonal anti-TUFM

TUFM Monoclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2A10D2

N° de cat : 67802-1-PBS

Synonymes

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

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Galerie de données de validation

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  WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

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  WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 67802-1-Ig (same clone as 67802-1-PBS)

  IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67802-1-PBS cible TUFM dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TUFM Protéine recombinante Ag24976
• Nom complet: Tu translation elongation factor, mitochondrial
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC010041
• Symbole du gène: TUFM
• Identification du gène (NCBI): 7284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

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