Anticorps Polyclonal de lapin anti-TUSC3

• Phare
• Validé par KD/KO

TUSC3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16039-1-AP

Synonymes

M33, MRT7, N33, OST3A, Protein N33, tumor suppressor candidate 3, TUSC3

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Galerie de données de validation

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  WB analysis of HeLa using 16039-1-AP

  WB result of TUSC3 antibody (16039-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-TUSC3 transfected HeLa cells.

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  WB analysis using 16039-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16039-1-AP (TUSC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 16039-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 16039-1-AP (TUSC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de souris, tissu testiculaire de rat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

16039-1-AP cible TUSC3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TUSC3 Protéine recombinante Ag8912
• Nom complet: tumor suppressor candidate 3
• Masse moléculaire calculée: 347 aa, 40 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC010370
• Symbole du gène: TUSC3
• Identification du gène (NCBI): 7991
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TUSC3 (tumor suppressor candidate 3), originally named N33, is a potential tumor supressor gene. Decreased expression of TUSC3 has been found in various cancers, including prostate cancer, pancreas cancer and ovary cancer. TUSC3 also known as OST3A, is identified as a part of the oligosaccharyl-transferase (OST) complex and plays a crucial role in protein N-glycosylation. TUSC3 mutations have been found in families with non-syndromic autosomal recessive mental retardation.

Publications

Species	Application	Title
		Nat Commun; miRNA-mediated TUSC3 deficiency enhances UPR and ERAD to promote metastatic potential of NSCLC.; Authors - Young-Jun Jeon
human	WB	J Cell Biol; Oxidoreductase activity is necessary for N-glycosylation of cysteine-proximal acceptor sites in glycoproteins.; Authors - Natalia A Cherepanova ; KD Validated
human	WB	Sci Rep; Mammalian cells lacking either the cotranslational or posttranslocational oligosaccharyltransferase complex display substrate-dependent defects in asparagine linked glycosylation.; Authors - Natalia A Cherepanova ; KD Validated
human	WB	J Biol Chem; ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence; Authors - Tetsuya Hirata
human	IHC	J Transl Med; Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma progression through LIPC/AKT axis; Authors - Ruxia Deng ; KD Validated
human	WB	J Allergy Clin Immunol; Epigenetic activation of the TUSC3 gene as a potential therapy for XMEN disease; Authors - Haodong Ding