Anticorps Polyclonal de lapin anti-TXNDC5

• Phare
• Validé par KD/KO

TXNDC5 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 19834-1-AP

Synonymes

EndoPDI, ER protein 46, ERP46, Hcc 2, PDIA15, Thioredoxin like protein p46, TLP46, TXNDC5, UNQ364

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Galerie de données de validation

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  WB analysis using 19834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19834-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19834-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 19834-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 19834-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 19834-1-AP (TXNDC5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 19834-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19834-1-AP (TXNDC5 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 19834-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TXNDC5 antibody (19834-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules COLO 320, tissu de côlon de souris, tissu de muscle squelettique humain, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer de l'endomètre humain, tissu de cancer du côlon humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:10000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

19834-1-AP cible TXNDC5 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TXNDC5 Protéine recombinante Ag13808
• Nom complet: thioredoxin domain containing 5 (endoplasmic reticulum)
• Masse moléculaire calculée: 432 aa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC001199
• Symbole du gène: TXNDC5
• Identification du gène (NCBI): 81567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IF,IP	Sci Adv; Targeting mechanosensitive endothelial TXNDC5 to stabilize eNOS and reduce atherosclerosis in vivo.; Authors - Chih-Fan Yeh ; KD Validated
human,mouse	WB,IHC,IF	Nat Commun; Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization.; Authors - Tzu-Han Lee ; KO Validated
mouse	IHC,IF,WB,CoIP,FC	J Clin Invest; Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGFβ signaling in kidney fibroblasts.; Authors - Yen-Ting Chen ; KD Validated
mouse	WB,CoIP	Int J Mol Sci; Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation in the Hepatic AML12 Cell Line; Authors - Seyed Hesamoddin Bidooki
mouse	WB	Antioxidants (Basel); Counteracting Cisplatin-Induced Testicular Damages by Natural Polyphenol Constituent Honokiol.; Authors - Tse-En Wang
mouse	WB	Antioxidants (Basel); Squalene Loaded Nanoparticles Effectively Protect Hepatic AML12 Cell Lines against Oxidative and Endoplasmic Reticulum Stress in a TXNDC5-Dependent Way.; Authors - Seyed Hesamoddin Bidooki