Anticorps Polyclonal de lapin anti-TXNIP

• Phare
• Validé par KD/KO

TXNIP Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

67

N° de cat : 18243-1-AP

Synonymes

EST01027, HHCPA78, THIF, Thioredoxin binding protein 2, TXNIP, VDUP1

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Galerie de données de validation

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  WB analysis of K-562 using 18243-1-AP

  WB result of TXNIP antibody (18243-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNIP transfected K-562 cells.

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  WB analysis of HL-60 using 18243-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 18243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18243-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18243-1-AP (TXNIP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human ovary tumor using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18243-1-AP (TXNIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse kidney using 18243-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18243-1-AP (TXNIP Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules K-562, cellules MCF-7, cellules PC-12
• Résultats positifs en IHC: tissu rénal humain, tissu de tumeur ovarienne humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 55 publications below
• IHC: See 18 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

18243-1-AP cible TXNIP dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TXNIP Protéine recombinante Ag13009
• Nom complet: thioredoxin interacting protein
• Masse moléculaire calculée: 391 aa, 44 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC093702
• Symbole du gène: TXNIP
• Identification du gène (NCBI): 10628
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TXNIP, also known as VDUP-1 or TBP-2, belongs to alpha-arrestin protein family and is perhaps the only family member known to bind thioredoxin (TRX). TXNIP was induced by Vitamin D3, but not induced by another monocyte or macrophage differentiation inducer: phorbol 12-myristate 13-acetate (PMA). TXNIP bound catalytic active center of thioredoxin (TRX), which protected cells against oxidative stress. TXNIP was found to be a negative regulator of thioredoxin activity and inducer of the intracellular level of reactive oxygen species (ROS). TXNIP plays an important role in a wide variety of biological functions, such as the regulation of cell death, growth, differentiation, and energy metabolism.

Publications

Species	Application	Title
mouse	FC	Gastroenterology; MondoA-Thioredoxin-Interacting Protein Axis Maintains Regulatory T-Cell Identity and Function in Colorectal Cancer Microenvironment.; Authors - Ying Lu
human	WB	Clin Cancer Res; FBW7 (F-box and WD repeat domain-containing 7) negatively regulates glucose metabolism by targeting the c-Myc/TXNIP (thioredoxin binding protein) axis in pancreatic cancer.; Authors - Shunrong Ji
mouse	IHC	Theranostics; Epigenetic repression of miR-17 contributed to di(2-ethylhexyl) phthalate-triggered insulin resistance by targeting Keap1-Nrf2/miR-200a axis in skeletal muscle.; Authors - Jie Wei
human	WB	Redox Biol; PMID: 32330868; Authors - Yifan Jia ; KD Validated
rat	WB	Oxid Med Cell Longev; PMID: 31998437; Authors - Jing Yu
mouse	WB,IF	Oxid Med Cell Longev; PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.; Authors - Zeyu Li

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