Anticorps Monoclonal anti-TXNRD1

• Phare
• Validé par KD/KO

TXNRD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B10C4

N° de cat : 67728-1-PBS

Synonymes

TRXR1, 1B10C4, EC:1.11.1.2, EC:1.8.1.9, Gene associated with retinoic and IFN-induced mortality 12 protein

---

Galerie de données de validation

• 

  WB analysis using 67728-1-Ig (same clone as 67728-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67728-1-Ig (TXNRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67728-1-Ig (same clone as 67728-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67728-1-Ig (TXNRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67728-1-Ig (same clone as 67728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67728-1-Ig (TXNRD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67728-1-Ig (same clone as 67728-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67728-1-Ig (TXNRD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67728-1-Ig (same clone as 67728-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TXNRD1 antibody (67728-1-Ig, Clone: 1B10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67728-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67728-1-PBS cible TXNRD1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TXNRD1 Protéine recombinante Ag30071
• Nom complet: thioredoxin reductase 1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 50-70 kDa
• Numéro d’acquisition GenBank: BC018122
• Symbole du gène: TXNRD1
• Identification du gène (NCBI): 7296
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Thioredoxin reductase-1 (TXNRD1) is uniquely capable of utilizing electrons from NADPH to recover the reduced state of TRX1. Interestingly, TXNRD1 is upregulated in many human malignancies and promotes cancer progression, and attenuation of TXNRD1 levels effectively suppresses the growth of tumor cells (PMID: 31384178). TXNRD1 is also upregulated in many human malignancies and functions as a prognostic factor for many tumors, such as oral squamous cell carcinomas, lung cancer, breast cancer, and astrocytomas. TXNRD1 protein levels were analyzed by inmmunoblotting. Bands of ~55 kDa for TXNRD1 were observed. IHC analysis suggested TXNRD1 was mainly located in the cytoplasm. (PMID: 28536696, PMID: 20584310)

{{ptg:RelatedPrimaryAntibodies}}