Anticorps Recombinant de lapin anti-TYK2

• Phare
• Validé par KD/KO

TYK2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230176E7

N° de cat : 83161-4-PBS

Synonymes

230176E7, EC:2.7.10.2, JTK1, tyrosine kinase 2

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Galerie de données de validation

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  WB analysis using 83161-4-RR (same clone as 83161-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83161-4-RR (TYK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83161-4-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83161-4-RR (same clone as 83161-4-PBS)

  WB result of TYK2 antibody (83161-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TYK2 transfected HEK-293 cells. This data was developed using the same antibody clone with 83161-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83161-4-RR (same clone as 83161-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83161-4-RR (TYK2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83161-4-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 83161-4-RR (same clone as 83161-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (83161-4-RR, Clone: 230176E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83161-4-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83161-4-RR (same clone as 83161-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug TYK2 Recombinant antibody (83161-4-RR, Clone:230176E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83161-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83161-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83161-4-RR against Human TYK2 were performed. The affinity constant is 0.116 nM.

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Informations sur le produit

83161-4-PBS cible TYK2 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TYK2 Protéine recombinante Ag28457
• Nom complet: tyrosine kinase 2
• Masse moléculaire calculée: 1187 aa, 134 kDa
• Poids moléculaire observé: 134 kDa
• Numéro d’acquisition GenBank: BC014243
• Symbole du gène: TYK2
• Identification du gène (NCBI): 7297
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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