Anticorps Monoclonal anti-Thymidylate synthase

• Phare
• Validé par KD/KO

Thymidylate synthase Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

1F5B7

Avis (1)

N° de cat : 66725-1-Ig

Synonymes

TYMS, 1F5B7, DKCD, EC:2.1.1.45, HsT422

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Galerie de données de validation

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  WB analysis using 66725-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66725-1-Ig (Thymidylate synthase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66725-1-Ig

  WB result of Thymidylate synthase antibody (66725-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thymidylate synthase transfected HEK-293 cells.

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  IHC staining of human colon cancer using 66725-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (Thymidylate synthase antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66725-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (Thymidylate synthase antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66725-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (Thymidylate synthase antibody) at dilution of 1:400 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66725-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66725-1-Ig (Thymidylate synthase antibody) at dilution of 1:400 (under 40x lens). Proteolytic pre-treatment mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66725-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66725-1-Ig (Thymidylate synthase antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66725-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Thymidylate synthase antibody (66725-1-Ig, Clone: 1F5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66725-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TYMS Monoclonal antibody (66725-1-Ig, Clone:1F5B7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66725-1-Ig cible Thymidylate synthase dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Thymidylate synthase Protéine recombinante Ag7027
• Nom complet: thymidylate synthetase
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC002567
• Symbole du gène: TYMS
• Identification du gène (NCBI): 7298
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Publications

Species	Application	Title
human	WB	J Med Chem; Fuplatin: An Efficient and Low-Toxic Dual-Prodrug; Authors - Ran Zhang
human	WB	Biochem Pharmacol; Design, synthesis and biological evaluation of novel curcumin-fluorouracil hybrids as potential anti-cancer agents; Authors - Xiaotong Wang
human	IF	Front Immunol; Single-cell sequencing analysis reveals cancer-associated pericyte subgroup in esophageal squamous cell carcinoma to predict prognosis; Authors - Kai Xiong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (03-26-2021)

Not working

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver, mouse brain / HepG2

Thymidylate synthase Antibody Western Blot validation (1:1000 dilution) in Mouse liver, mouse brain / HepG2 (Cat no:66725-1-Ig)

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