Anticorps Polyclonal de lapin anti-TYRO3

TYRO3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28513-1-PBS

Synonymes

Brt, BYK, Dtk, EC:2.7.10.1, RSE

---

Galerie de données de validation

• 

  WB analysis using 28513-1-AP (same clone as 28513-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28513-1-AP (TYRO3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 28513-1-AP (same clone as 28513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 28513-1-AP (same clone as 28513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28513-1-AP (same clone as 28513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28513-1-AP (same clone as 28513-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HCT 116 using 28513-1-AP (same clone as 28513-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TYRO3 antibody (28513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

28513-1-PBS cible TYRO3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TYRO3 Protéine recombinante Ag29540
• Nom complet: TYRO3 protein tyrosine kinase
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC049368
• Symbole du gène: TYRO3
• Identification du gène (NCBI): 7301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TYRO3 has a structure includes a cytoplasmic domain and an extracellular ligand binding region. And ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. TYRO3 is associated with many physiological processes including cell survival, migration and differentiation. It has been reported that TYRO3 can be expressed in various regions of the mammalian central nervous system. 28513-1-AP antibody recognizes the 97 kDa and 140 kDa protein which might be the glycosylated form similar to the papers.((PMID: 8873131, 23354874, 21539887)

{{ptg:RelatedPrimaryAntibodies}}