Anticorps Polyclonal de lapin anti-TYROBP/DAP12

TYROBP/DAP12 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 28138-1-AP

Synonymes

TYROBP, KAR-associated protein, KARAP, KAR associated protein, DNAX-activation protein 12

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Galerie de données de validation

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  WB analysis using 28138-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 28138-1-AP (TYROBP / DAP12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules THP-1,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

28138-1-AP cible TYROBP/DAP12 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TYROBP/DAP12 Protéine recombinante Ag27252
• Nom complet: TYRO protein tyrosine kinase binding protein
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 10-12 kDa
• Numéro d’acquisition GenBank: BC011175
• Symbole du gène: TYROBP
• Identification du gène (NCBI): 7305
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TYROBP, also known as DAP12 or KARAP, is a transmembrane protein consisting of a very small extracellular region, a transmembrane domain containing an aspartic acid residue critical for association with its partner subunits, and an intracellular domain with a single immunoreceptor tyrosine-based activation motif (ITAM) (PMID: 11114420). It is expressed as a disulfide-bonded homodimer on natural killer cells, myeloid cells and a subset of T cells (PMID: 11114420). TYROBP acts as a signaling adaptor that provides signaling function via the Syk and ZAP-70 tyrosine kinase activation pathways (PMID: 15895090). The gene of TYROBP is located on the long arm of chromosome 19 at position 13.1. Multiple alternative transcript variants encoding distinct isoforms have been identified for this gene.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Berberine modulates microglial polarization by activating TYROBP in Alzheimer's disease; Authors - Yu Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katia (Verified Customer) (09-09-2025)

The antibody performed well on mouse brain homogenate. However, it did not yield a signal in primary cells homogenate, possibly due to lower protein loading. Notably, a different DAP12 antibody worked under the same conditions.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain - mouse primary cells

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