Anticorps Recombinant de lapin anti-Talin-1

Talin-1 Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

7D11

N° de cat : 82856-4-PBS

Synonymes

7D11, ILWEQ, KIAA1027, talin 1, talin1

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Galerie de données de validation

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  WB analysis using 82856-4-RR (same clone as 82856-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82856-4-RR (Talin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82856-4-RR (same clone as 82856-4-PBS)

  IP result of anti-Talin-1 (IP:82856-4-RR, 4ug; Detection:82856-4-RR 1:3000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 82856-4-RR (same clone as 82856-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 82856-4-RR (same clone as 82856-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82856-4-RR (same clone as 82856-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82856-4-RR (same clone as 82856-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82856-4-RR (Talin-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 82856-4-RR (same clone as 82856-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (82856-4-RR, Clone: 7D11 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 82856-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82856-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82856-4-RR against Human TLN1 were performed. The affinity constant is 0.215 nM.

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Informations sur le produit

82856-4-PBS cible Talin-1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Talin-1 Protéine recombinante Ag5367
• Nom complet: talin 1
• Masse moléculaire calculée: 2541 aa, 270 kDa
• Poids moléculaire observé: 270 kDa
• Numéro d’acquisition GenBank: BC042923
• Symbole du gène: Talin-1
• Identification du gène (NCBI): 7094
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

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