Anticorps Polyclonal de lapin anti-Tenascin-X

Tenascin-X Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13595-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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  WB analysis using 13595-1-AP (same clone as 13595-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-1080 using 13595-1-AP (same clone as 13595-1-PBS)

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

  IP result of anti-Tenascin-X (IP:13595-1-AP, 4ug; Detection:13595-1-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 13595-1-AP (same clone as 13595-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13595-1-PBS cible Tenascin-X dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Tenascin-X Protéine recombinante Ag4501
• Nom complet: tenascin XB
• Masse moléculaire calculée: 4289 aa, 464 kDa
• Poids moléculaire observé: 75 kDa, 140 kDa
• Numéro d’acquisition GenBank: BC033740
• Symbole du gène: TNXB
• Identification du gène (NCBI): 7148
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tenascin-X (TNXB) is an extracellular matrix glycoprotein predominantly located in the outer reticular lamina of the basement membrane (BM) (PMID: 23768946). It interacts with many other ECM proteins and it accelerates collagen fibrillogenesis in vitro. TNXB plays a role in interactions between cell and ECM, antiadhesive effect, inhibiting cell migration, and in maintaining homeostasis of the extracellular matrix. Deficiency of TNXB has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This antibody is raised against human TNXB and detects a band of about 75 kDa, which probably represents a proteolytic cleaved form of human TNXB (PMID: 17263730). But the serum form of tenascin-X is a 140-kD protein probably resulting from proteolytic cleavage or alternative splicing of tenascin-X(PMID: 16567571, 11642233).

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