Anticorps Recombinant de lapin anti-Thioredoxin

• Phare
• Validé par KD/KO

Thioredoxin Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

7G10

N° de cat : 82765-1-PBS

Synonymes

TXN, 7G10, ADF, ATL-derived factor, Hom s Trx

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Galerie de données de validation

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  WB analysis using 82765-1-RR (same clone as 82765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82765-1-RR (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82765-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 82765-1-RR (same clone as 82765-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82765-1-RR (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82765-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 82765-1-RR (same clone as 82765-1-PBS)

  WB result of Thioredoxin antibody (82765-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thioredoxin transfected A549 cells. This data was developed using the same antibody clone with 82765-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 82765-1-RR (same clone as 82765-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82765-1-RR (Thioredoxin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82765-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82765-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82765-1-RR against Human Thioredoxin were performed. The affinity constant is 0.437 nM.

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Informations sur le produit

82765-1-PBS cible Thioredoxin dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Thioredoxin Protéine recombinante Ag6989
• Nom complet: thioredoxin
• Masse moléculaire calculée: 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC003377
• Symbole du gène: TXN
• Identification du gène (NCBI): 7295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

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