Anticorps Recombinant de lapin anti-CD141/Thrombomodulin

CD141/Thrombomodulin Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240365D1

N° de cat : 83416-4-RR

Synonymes

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

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Galerie de données de validation

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  WB analysis using 83416-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83416-4-RR (Thrombomodulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 83416-4-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 83416-4-RR

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 83416-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83416-4-RR (CD141/Thrombomodulin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung squamous cell carcinoma using 83416-4-RR

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83416-4-RR (CD141/Thrombomodulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical squamous cancer using 83416-4-RR

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83416-4-RR (CD141/Thrombomodulin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 83416-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 83416-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83416-4-RR (Thrombomodulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 83416-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Thrombomodulin antibody (83416-4-RR, Clone: 240365D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83416-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83416-4-RR against Human CD141/Thrombomodulin were performed. The affinity constant is 1.15 nM.

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules A431
• Résultats positifs en IHC: tissu pulmonaire humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83416-4-RR cible CD141/Thrombomodulin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: thrombomodulin
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: NM_000361.3
• Symbole du gène: THBD
• Identification du gène (NCBI): 7056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

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