Anticorps Polyclonal de lapin anti-Tie-2/CD202b

Tie-2/CD202b Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19157-1-PBS

Synonymes

TEK, Angiopoietin 1 receptor, Angiopoietin-1 receptor, CD202B, EC:2.7.10.1

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Galerie de données de validation

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  WB analysis of mouse lung using 19157-1-AP (same clone as 19157-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie-2/CD202b antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 19157-1-AP (same clone as 19157-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 19157-1-AP (same clone as 19157-1-PBS)

  IP result of anti-Tie2 (IP:19157-1-AP, 4ug; Detection:19157-1-AP 1:500) with mouse lung tissue lysate 4000ug. This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 19157-1-AP (same clone as 19157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 19157-1-AP (same clone as 19157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 19157-1-AP (same clone as 19157-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19157-1-AP (Tie2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19157-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19157-1-PBS cible Tie-2/CD202b dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Tie-2/CD202b Protéine recombinante Ag13523
• Nom complet: TEK tyrosine kinase, endothelial
• Masse moléculaire calculée: 1124 aa, 126 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC035514
• Symbole du gène: Tie2
• Identification du gène (NCBI): 7010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tie2 (also known as TEK) is a tyrosine-protein kinase expressed almost exclusively on endothelial cells. It contains two immunoglobulin-like domains, three epidermal growth factor (EGF)--like domains and three fibronectin type III repeats. Tie2 acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Mutations in the gene Tie2 are associated with inherited venous malformations of the skin and mucous membranes. Human Tie2 has a calculated molecular weight of 126 kDa. As a result of glycosylation, the apparent molecular mass of Tie2 is approximately 140-160 kDa.

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