Anticorps Monoclonal anti-TIM23

TIM23 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3H10F5

N° de cat : 67535-1-PBS

Synonymes

67535, TIMM23, 3H10F5, Mitochondrial import inner membrane translocase subunit Tim23, TIMM 23

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Galerie de données de validation

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  WB analysis using 67535-1-Ig (same clone as 67535-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67535-1-Ig (TIM23 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (TIM23 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67535-1-Ig (same clone as 67535-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67535-1-Ig (TIM23 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67535-1-Ig (same clone as 67535-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TIM23 antibody (67535-1-Ig, Clone: 3H10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67535-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67535-1-PBS cible TIM23 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TIM23 Protéine recombinante Ag27069
• Nom complet: translocase of inner mitochondrial membrane 23
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC062707
• Symbole du gène: TIMM23
• Identification du gène (NCBI): 100287932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tim23 is a mitochondrial inner membrane protein essential for import of preproteins into the matrix. Tim23 together with Tim17 and Tim44 form the Tim23 complex which mediates the import of nuclear encoded preproteins into the mitochondria. Tim23 is commonly used as loading control for mitochondrial inner membrane protein.

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