Anticorps Recombinant de lapin anti-Timp-3

Timp-3 Recombinant Antibody for ELISA, WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

ELISA, WB

Conjugaison

Non conjugué

CloneNo.

240374D8

N° de cat : 83627-1-PBS

Synonymes

TIMP3, Protein MIG 5, Metalloproteinase inhibitor 3, K222TA2, K222

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Galerie de données de validation

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  WB analysis of human placenta using 83627-1-RR (same clone as 83627-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 83627-1-RR (Timp-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MDA-MB-231 using 83627-1-RR (same clone as 83627-1-PBS)

  1x10^6 MDA-MB-231 cells were intracellularly stained with 0.25 ug Timp-3 Recombinant antibody (83627-1-RR, Clone:240374D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83627-1-RR (same clone as 83627-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Timp-3 Recombinant antibody (83627-1-RR, Clone:240374D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83627-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83627-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83627-1-RR against Human Timp-3 were performed. The affinity constant is 2.63 nM.

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Informations sur le produit

83627-1-PBS cible Timp-3 dans les applications de ELISA, WB et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Timp-3 Protéine recombinante Ag34657
• Nom complet: TIMP metallopeptidase inhibitor 3
• Masse moléculaire calculée: 24 kDa
• Numéro d’acquisition GenBank: BC014277
• Symbole du gène: TIMP3
• Identification du gène (NCBI): 7078
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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