Anticorps Recombinant de lapin anti-Tissue Factor/CD142

Tissue Factor/CD142 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240875H3

N° de cat : 83887-4-RR

Synonymes

CD142, Coagulation factor III, F3, Tissue factor, 240875H3

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Galerie de données de validation

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  WB analysis using 83887-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 83887-4-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83887-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83887-4-RR against Mouse Tissue Factor were performed. The affinity constant is 0.85 nM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules NIH/3T3, cellules SH-SY5Y, tissu cérébral de souris, tissu cutané de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83887-4-RR cible Tissue Factor/CD142 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Tissue Factor/CD142 Protéine recombinante Eg1102
• Nom complet: coagulation factor III
• Masse moléculaire calculée: 33kDa
• Poids moléculaire observé: 48-53 kDa
• Symbole du gène: Tissue Factor
• Identification du gène (NCBI): 14066
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108). This antibody is specific for mouse Tissue Factor.

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