Anticorps Polyclonal de lapin anti-Titin

Titin Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (2)

N° de cat : 27867-1-AP

Synonymes

TTN

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Galerie de données de validation

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  IHC staining of mouse skeletal muscle using 27867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 27867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 27867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skeletal muscle using 27867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 27867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27867-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 27867-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Titin antibody (27867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu cardiaque de souris, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de muscle squelettique de souris, tissu cardiaque de souris
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 6 publications below

Informations sur le produit

27867-1-AP cible Titin dans les applications de IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Titin Protéine recombinante Ag27496
• Nom complet: titin
• Masse moléculaire calculée: 3816 kDa
• Numéro d’acquisition GenBank: NM_001256850
• Symbole du gène: Titin
• Identification du gène (NCBI): 7273
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Titin, or connectin, is a giant muscle protein expressed in the cardiac and skeletal muscles that spans half of the sarcomere from Z line to M line. It plays a key role in muscle contraction and relaxation, maintaining the structural integrity of the myotome(PMID: 11846417). Mutations in Titin are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma(PMID: 15802564).

Publications

Species	Application	Title
mouse	IF	Elife; A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation.; Authors - Jie Wang
mouse	IF	Dev Biol; Aberrant differentiation of second heart field mesoderm prefigures cellular defects in the outflow tract in response to loss of FGF8; Authors - Sophie Astrof
mouse	IF	Res Sq; Titin is a nucleolar protein in neurons; Authors - Lynn George
human	IF	Cell Stem Cell; Generation of self-organized neuromusculoskeletal tri-tissue organoids from human pluripotent stem cells; Authors - Yao Yin
mouse	IF	Physiol Rep; Germline deletion of Rgs2 and/or Rgs5 in male mice does not exacerbate left ventricular remodeling induced by subchronic isoproterenol infusion; Authors - Shelby Dahlen
human	IF	Stem Cell Res; Generation of a human induced pluripotent stem cell line YCMi004-A from a patient with dilated cardiomyopathy carrying a protein-truncating mutation of the Titin gene and its differentiation towards cardiomyocytes; Authors - Sun-Ho Lee

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Víctor (Verified Customer) (01-26-2023)

The antibody is a bit dirty but specific for muscle cells. I'm trying to evaluate specific sarcomeric structures with higher magnifications, and they seem to be marked. So the antibody is OK.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Skeletal muscle cells obtained by direct cell reprogramming approaches

Titin Antibody Immunofluorescence validation (1:400 dilution) in Skeletal muscle cells obtained by direct cell reprogramming approaches (Cat no:27867-1-AP)

FH

Joseph (Verified Customer) (01-14-2020)

I tested the Titin antibody using IHC on 16uM mouse skeletal muscle sections (Tibialis anterior and Soleus) using 1:100 and 1:300 dilutions. Both dilutions worked very well.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Mouse Skeletal Muscle - Soleus, tibialis anterior

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