Anticorps Polyclonal de lapin anti-TLR4

TLR4 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 30400-1-AP

Synonymes

CD284, toll like receptor 4

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Galerie de données de validation

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  WB analysis using 30400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30400-1-AP (TLR4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30400-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30400-1-AP (TLR4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: J774A.1 cells, cellules RAW 264.7, cellules THP-1, tissu hépatique de rat, tissu hépatique de souris, tissu splénique de rat, tissu splénique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Informations sur le produit

30400-1-AP cible TLR4 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TLR4 Protéine recombinante Ag32998
• Nom complet: toll-like receptor 4
• Masse moléculaire calculée: 96 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: NM_021297
• Symbole du gène: Tlr4
• Identification du gène (NCBI): 21898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TLR4, also known as CD284, belongs to the Toll-like receptor family. It is a type I transmembrane protein consisting of an extracellular leucine-rich repeat region, a helix transmembrane domain, and an intracellular Toll-IL-1 receptor domain. TLR4 functions as a pattern recognition receptor recognizing pathogen- and damage-associated molecular patterns (PAMPs and DAMPs) to induce innate immune responses via downstream signaling pathways. It interacts with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). TLR4 acts via MYD88, TIRAP, and TRAF6, leading to NF-kB activation, cytokine secretion, and the inflammatory response.

Publications

Species	Application	Title
mouse	WB,IF	Free Radic Biol Med; Short-term exposure to low doses of aflatoxin B1 aggravates nonalcoholic steatohepatitis by TLR4-mediated necroptosis; Authors - Junya Zeng
mouse	WB	Brain Behav Immun Health; Interleukin-18 interacts with NKCC1 to mediate brain injury after intracerebral hemorrhage; Authors - Beibei Xu
rat,mouse	WB	Heliyon; Asprosin promotes vascular inflammation via TLR4-NFκB-mediated NLRP3 inflammasome activation in hypertension; Authors - Rui Ge
mouse	WB	ACS Appl Mater Interfaces; Clinical Grade Fibroin Sutures with Bioactive Gold Clusters Enhance Surgical Wound Healing via Inflammation Modulation; Authors - Fangzhen Tian
mouse	WB	Int J Immunopathol Pharmacol; Corilagin relieves atherosclerosis via the toll-like receptor 4 signaling pathway in vascular smooth muscle cells; Authors - Yujie Wang ; KD Validated
mouse	WB	Adv Healthc Mater; Atomic Artificial Enzyme for Acute and Chronic Pneumonia; Authors - Wei Liu

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