Anticorps Recombinant de lapin anti-TNF-alpha

TNF-alpha Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240702B7

N° de cat : 80258-6-RR

Synonymes

Tnf, Tnfa, TNF alpha,TNF-a,TNF-alpha,TNFα, TNF alpha, TNF a

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 80258-6-RR

  PMA, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80258-6-RR (Tnfa antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 80258-6-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Tnfa antibody (80258-6-RR, Clone: 240702B7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 80258-6-RR

  Biolayer interferometry (BLl) kinetic assays of 80258-6-RR against Mouse Tnfa were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: PMA, LPS and Brefeldin A treated RAW 264.7 cells,
• Résultats positifs en IF/ICC: cellules RAW 264.7 traitées au LPS et à la bréfeldine A,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

80258-6-RR cible TNF-alpha dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: tumor necrosis factor
• Poids moléculaire observé: 25-26 kDa
• Numéro d’acquisition GenBank: NM-013693
• Symbole du gène: TNF-alpha
• Identification du gène (NCBI): 21926
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Mouse and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the mouse form is glycosylated. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.

Publications

Species	Application	Title
mouse	IF	Adv Healthc Mater; Structured Polymers Enable the Sustained Delivery of Glucocorticoids within the Intra-Articular Space; Authors - Ana Crastin

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