Anticorps Monoclonal anti-TOM22

TOM22 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1C2B11

N° de cat : 66562-1-PBS

Synonymes

TOMM22, 1C2B11, 1C9 2, hTom22, MST065

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Galerie de données de validation

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  WB analysis using 66562-1-Ig (same clone as 66562-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66562-1-Ig (Tom22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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  WB analysis using 66562-1-Ig (same clone as 66562-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66562-1-Ig (Tom22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66562-1-Ig (TOMM22 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66562-1-Ig (Tom22 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66562-1-Ig (same clone as 66562-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Tom22 antibody (66562-1-Ig, Clone: 1C2B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 66562-1-Ig (same clone as 66562-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Tom22 (66562-1-Ig, Clone:1C2B11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66562-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66562-1-PBS cible TOM22 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TOM22 Protéine recombinante Ag1797
• Nom complet: translocase of outer mitochondrial membrane 22 homolog (yeast)
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC009363
• Symbole du gène: TOMM22/Tom22
• Identification du gène (NCBI): 56993
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tom22 is a central receptor component of the translocase of the outer membrane of mitochondria responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. It is showed in UniProt that phosphorylation is performed at three positions of this protein which may increase the molecular weight. Catalog#66562-1-Ig recognises  the  22 kDa  protein larger than the calculated MW which is 15 kDa. Besides, it has been reported the MV of TOMM22 is 22 kDa (PMID:19285029).

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