Anticorps Monoclonal anti-Transferrin/TF

Transferrin/TF Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2B4C6

N° de cat : 66171-1-PBS

Synonymes

TF, transferrin, 2B4C6, Beta 1 metal binding globulin, Beta-1 metal-binding globulin

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Galerie de données de validation

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  WB analysis of human plasma using 66171-1-Ig (same clone as 66171-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66171-1-Ig (same clone as 66171-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 66171-1-Ig (same clone as 66171-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66171-1-PBS cible Transferrin/TF dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Transferrin/TF Protéine recombinante Ag11668
• Nom complet: transferrin
• Masse moléculaire calculée: 698 aa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC059367
• Symbole du gène: TF
• Identification du gène (NCBI): 7018
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273

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