Anticorps Recombinant de lapin anti-TrkB

TrkB Recombinant Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

241473G5

Avis (1)

N° de cat : 80878-6-RR

Synonymes

NTRK2, 241473G5, BDNF/NT-3 growth factors receptor, GP145 TrkB, GP145-TrkB

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Galerie de données de validation

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  WB analysis using 80878-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80878-6-RR (TrkB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse cerebellum using 80878-6-RR

  IP result of anti-TrkB (IP:80878-6-RR, 4ug; Detection:80878-6-RR 1:4000) with mouse cerebellum tissue lysate 2000 ug.

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  IHC staining of rat brain using 80878-6-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80878-6-RR (TrkB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 80878-6-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80878-6-RR (TrkB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 80878-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TrkB antibody (80878-6-RR, Clone: 241473G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 80878-6-RR

  Biolayer interferometry (BLl) kinetic assays of 80878-6-RR against Human TrkB were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IP: tissu de cervelet de souris,
• Résultats positifs en IHC: tissu cérébral de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80878-6-RR cible TrkB dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TrkB Protéine recombinante Ag3790
• Nom complet: neurotrophic tyrosine kinase, receptor, type 2
• Masse moléculaire calculée: 822 aa, 92 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: BC031835
• Symbole du gène: TrkB
• Identification du gène (NCBI): 4915
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tropomyosin-related kinase B (TrkB) is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. It is a single-pass type 1 membrane protein consisting of three domains: an extracellular ligand binding domain, a transmembrane domain, and an intracellular tyrosine kinase domain. TrkB is primarily expressed in the central and peripheral nervous systems. TrkB plays an important role in neural development and maintenance by regulating neuronal differentiation, proliferation, survival, migration, neurogenesis, and plasticity. This antibody raised against 41-433aa of human TrkB recognizes the full-length form (140-145 kDa) and truncated form (90-95 kDa) of TrkB (PMID: 23526925; 21039416).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (09-24-2025)

TRKB (in green) worked perfectly in the human TSC FFPE cortical tissue

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

TrkB Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:80878-6-RR)

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