Anticorps Polyclonal de lapin anti-Tuberin/TSC2

• Phare
• Validé par KD/KO

Tuberin/TSC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24601-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 24601-1-AP (same clone as 24601-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

  WB result of Tuberin/TSC2 antibody (24601-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  WB analysis using 24601-1-AP (same clone as 24601-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 24601-1-AP (same clone as 24601-1-PBS)

  IP result of anti-Tuberin/TSC2 (IP:24601-1-AP, 4ug; Detection:24601-1-AP 1:1000) with SH-SY5Y cells lysate 1080 ug. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 24601-1-AP (same clone as 24601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 24601-1-AP (same clone as 24601-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 24601-1-AP (same clone as 24601-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Tuberin/TSC2 antibody (24601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Tuberin (24601-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24601-1-PBS cible Tuberin/TSC2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Tuberin/TSC2 Protéine recombinante Ag18133
• Nom complet: tuberous sclerosis 2
• Masse moléculaire calculée: 1807 aa, 201 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC150300
• Symbole du gène: TSC2
• Identification du gène (NCBI): 7249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TSC2, also named as TSC4, FLJ43106 and LAM, acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In complex with TSC1, TSC2 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. TSC2 implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. TSC2 stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors.

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