Anticorps Monoclonal anti-Alpha Tubulin

Alpha Tubulin Monoclonal Antibody for WB

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, CoIP

Conjugaison

HRP

Publications

104

CloneNo.

1E4C11

N° de cat : HRP-66031

Synonymes

Alpha Tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, HRP Tubulin Alpha, K ALPHA 1, TUBA1B, tubulin, Tubulin Alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b, α-tubulin

---

Galerie de données de validation

• 

  WB analysis of multi-cells/tissue using HRP-66031

  Western blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody HRP-60031 at a dilution of 1:5000.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 97 publications below
• CoIP: See 1 publications below

Informations sur le produit

HRP-66031 cible Alpha Tubulin dans les applications de WB, CoIP et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: tubulin, alpha 1b
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC009314
• Symbole du gène: TUBA1B
• Identification du gène (NCBI): 10376
• Conjugaison: HRP
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the function of alpha-tubulin?

Alpha-tubulin belongs to a large superfamily of tubulin proteins. There are a number of different subtypes that have a molecular weight of ~50kDa and are able to bind to beta-tubulin, forming a heterodimer that polymerizes to microtubules as part of the cytoskeleton. These maintain cell structure, provide platforms for intracellular transport, and are also involved in cell division.

Where is alpha-tubulin expressed?

Alpha-tubulin is highly conserved and is present in nearly all eukaryotic cells as one of the building blocks of microtubules. The ubiquitous nature of this protein has led to its common use as a control protein for many tissue types as well as highlighting the structure of the cytoskeleton.

What are the post-translational modifications of alpha-tubulin?

The function and properties of microtubules are drastically affected by the post-translational modifications undergone by tubulin, which may occur to the tubulin dimer directly or to the polymerized microtubule. For example, the first modification to be identified was detyrosination,¹ as most alpha-tubulins have a tyrosine at their terminus. This process affects microtubules more than dimers and leads to patches of detyrosination along the structure, regulating protein interactions and allowing subcellular compartments to be defined.^2,3 Polyglutamylation also occurs at several sites within the carboxy-terminal tails. However, to date, the most-studied alpha tubulin modification is related to acetylation of lysine 40 (K40).

1. Gundersen, G. G., Khawaja, S. &  Bulinski, J. C. Postpolymerization detyrosination of alpha-tubulin: a  mechanism for subcellular differentiation of microtubules. J. Cell  Biol. 105, 251-64 (1987).

2. Galjart, N. Plus-End-Tracking Proteins  and Their Interactions at Microtubule Ends. Curr. Biol. 20,  R528-R537 (2010).

3. Jiang, K. & Akhmanova, A.  Microtubule tip-interacting proteins: a view from both ends. Curr.  Opin. Cell Biol. 23, 94-101 (2011).

Publications

Species	Application	Title
human	WB	Cell; Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.; Authors - Rui Yang
human	WB	Cell; Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.; Authors - Shicheng Su
rat	WB	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
human	WB	Immunity; Human Virus-Derived Small RNAs Can Confer Antiviral Immunity in Mammals.; Authors - Yang Qiu
mouse	WB	Immunity; Very-low-density lipoprotein receptor-enhanced lipid metabolism in pancreatic stellate cells promotes pancreatic fibrosis.; Authors - Xuguang Yang
mouse	WB	Nat Immunol; Control of CD1d-restricted antigen presentation and inflammation by sphingomyelin.; Authors - Espen Melum

Produits associés

Alpha Tubulin Monoclonal antibody

66031-1-Ig

Alpha Tubulin Polyclonal antibody

11224-1-AP

Beta Tubulin Polyclonal antibody

10094-1-AP

Beta Tubulin Polyclonal antibody

10068-1-AP

Beta Actin Monoclonal antibody

66009-1-Ig